Effect of surface modification of siliconeon Staphylococcus epidermidis adhesion and colonization.
نویسندگان
چکیده
Cerebrospinal fluid (CSF) shunts for the treatment of hydrocephalus are generally made of silicone rubber. The growth of bacterial colonies on the silicone surface leads to frequent CSF shunt complications. A systematic study of the effect of the surface modification of silicone on Staphylococcus epidermidis adhesion and colonization was performed for different incubation times by means of colony counting and scanning electron microscopy (SEM). Silicone was modified with different biopolymers and silanes, including heparin, hyaluronan, octadecyltrichlorosilane (OTS), and fluoroalkylsilane (FAS) to provide a stable and biocompatible surface with different surface functional groups and degrees of hydrophobicity. The modified silicone surfaces were studied by using contact angle measurements, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). After 4 and 8 h of incubation, the FAS- and OTS-coated silicone and the hyaluronan coated OTS/silicone surfaces showed significantly reduced bacterial adhesion and colonization compared to blank silicone by both quantification methods. However, the heparin coated OTS/silicone showed significantly increased bacterial adhesion. These results indicate that the nature of the surface functional group and surface roughness determine the extent of bacterial adhesion and colonization. However, the degree of hydrophobicity of the surface did not appear to play a determining role in bacterial adhesion and colonization.
منابع مشابه
Antibacterial Modification of Intravascular Catheter Surface for the Prevention of Catheter-Associated Infection
Objective: Intravascular catheter-associated infection has been increasing hospitalization in post-surgery patients mainly due to microbial colonization of the catheter surface and formation of a superficial biofilm layer. The present study is aimed in developing an effective antibacterial device which can prevent colonization of organisms by modification of catheter. Methods: In the present st...
متن کاملAntibacterial Modification of Intravascular Catheter Surface for the Prevention of Catheter-Associated Infection
Objective: Intravascular catheter-associated infection has been increasing hospitalization in post-surgery patients mainly due to microbial colonization of the catheter surface and formation of a superficial biofilm layer. The present study is aimed in developing an effective antibacterial device which can prevent colonization of organisms by modification of catheter. Methods: In the present st...
متن کاملPlasma can reduce Staphylococcus epidermidis biofilm formation on medical polymers
Medical polymers, such as urinary catheters are widely used biomaterials. One of the main problem for using the urinary catheters is biofilm formation on their surface, when they are used in a long time in the body. Virulence and pathogenicity of Staphylococcus epidermidis is often enhanced when growing as a biofilm. Many techniques have been presented to reduce the biofilm formation by surface...
متن کاملThe inhibition of Staphylococcus epidermidis biofilm formation by vancomycin-modified titanium alloy and implications for the treatment of periprosthetic infection.
Peri-prosthetic infections are notoriously difficult to treat as the biomaterial implant is ideal for bacterial adhesion and biofilm formation, resulting in decreased antibiotic sensitivity. Previously, we reported that vancomycin covalently attached to a Ti alloy surface (Vanc-Ti) could prevent bacterial colonization. Herein we examine the effect of this Vanc-Ti surface on Staphylococci epider...
متن کاملاصلاح سطح ابرآبگریز پلیمر پلیپروپیلن با هدف بهبود برهمکنشهای بیولوژیک
The significance of producing superhydrophobic surfaces through modification of surface chemistry and structure is in preventing or delaying biofilm formation. This is done to improve biocompatibility and chemical and biological properties of the surface by creating micro-nano multilevel rough structure; and to decrease surface free energy by Fault Tolerant Control Strategy (FTCS) . Here, we pr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of biomedical materials research. Part A
دوره 80 4 شماره
صفحات -
تاریخ انتشار 2007